We performed a transformation to test our antibiotics and to test our competent cells before we began. We used a PViB plasmid for our transformation, because PViB will cause the cells to glow, which is a easy way to see if our cells accepted the PViB plasmid in transformation. However, in transformation, bacteria grew on the control plate and the experiment plate. The bacteria shouldn't have grown on the control plate because the control bacteria don't have the plasmid. Along with PViB, the plasmid has an ampicillin resistance and our plates have ampicillin. Our control bacteria shouldn't have grown because they don't have the ampicillin resistance and shouldn't have grown on a plate with ampicillin. We didn’t know what it was due to, so we did another transformation to see what went wrong. We used nine plates. The first two plates were a repeat of the original transformation experiment. The next three plates had ampicillin added on top of the plates, because one thing that might have gone wrong in the original experiment was that the ampicillin might not have been distributed throughout the whole plate. However, the bacteria without the resistance plasmid grew on all five plates, meaning that our ampicillin doesn’t work. We also used two plates with Chloramphenicol, another antibiotic, and bacteria grew on the plate with PViB and not on the control plate, meaning that the Chloramphenicol is viable. Finally, we did two plates with DH5α, a type of competent cell, and no PViB gene, and bacteria grew on both plates, further confirming that our ampicillin doesn’t work.