Lab Notebook | ren-bio-builder

Thursday, April 28, 2016

Today, we're practicing Helicase PCR. We also ran one regular PCR so that we can compare the results of both.

We looked over our gels from last class today. The results were inconclusive. There was migration on our transformed gel, but not our...

We began today by working on our background section for our BioTrek paper. Then we completed the practice restriction digest that we...

We didn't really do a lot today; but we did plan for a restriction digest and gel on Wednesday.

We got lots of weird molds on our plates over break. Also, our Amp has stopped working again...

Today we transformed the cells from yesterday, using the PViB gene. We also finished up work on our BioTrek abstract which is due tomorrow.

Turns out that the frozen E-coli cells in the freezer aren't dead, because they grew in a nutrient broth, but for some reason, they did...

Last class we re-plated our "competent" E-coli cells, so that even if our E-coli cells aren't competent, we can still have a living...

Today we talked about our project goals and what the reason is for amplifying methylated DNA. Other procedures already exist to detect...

We began transformations of four different pars today: a promoter/RBS, an RFP gene, a GFP gene, and a terminator.